|
|
PRODUCTION-SCALE CHROMATOGRAPHY
(COCHR)
Format: Video
O V E R V I E W
This training unit is designed to introduce trainees to the equipment and operation of a production-scale chromatography system. After completing this unit, trainees should be able to describe the basic principles of production-scale chromatography. Trainees should also be able to identify the main parts of a typical production-scale chromatography system and describe its operation. In addition, trainees should be able to describe operator responsibilities and typical procedures for operating a production-scale chromatography system and for regenerating the sorbent.
O B J E C T I V E S
Basic Principles
- Define production-scale chromatography, solute, mobile phase, stationary phase, eluant, liquid chromatography, solvent, gas chromatography, carrier gas, and sorbent.
- Describe the basic principles of chromatographic separation.
Equipment - Part 1
- Identify the main parts of a typical production-scale chromatography system and describe their functions.
- Identify the four major steps in a chromatography cycle.
- Explain the basic difference between product cuts and transition cuts.
- Describe a three-step process for separating a strongly adsorbed component.
Equipment - Part 2
- Identify types of equipment associated with a typical chromatography column and describe their functions.
- Identify general types of product recovery equipment and purification equipment that may be used in production-scale chromatography systems.
Startup
- Identify the major parts of a typical production-scale liquid chromatography system.
- Define forecut, richcut, and tailcut.
- Describe operator responsibilities associated with taking cuts and maintaining column level in a typical production-scale liquid chromatography system.
- Describe a startup procedure for a typical production-scale liquid chromatography system.
Operation - Part 1
- Describe the major operating steps for a typical production-scale liquid chromatography system.
Operation - Part 2
- Describe operating procedures and operator responsibilities for a typical production- scale liquid chromatography system.
Regeneration - Part 1
- Define regenerant and reclassify.
- Describe the major steps for regenerating the resin bed in a typical production-scale liquid chromatography column.
Regeneration - Part 2
- Describe procedures for regenerating the resin bed in a typical production-scale liquid chromatography column.
- Describe procedures for replacing spent resin in a typical production-scale liquid chromatography column.
S U B J E C T S
Basic Principles
- Uses of production-scale chromatography
- Principles of chromatography
- Liquid chromatography and gas chromatography
- Sorbents
Equipment - Part 1
- Parts and operation of a typical production-scale chromatography system
- A chromatography cycle
- Cuts
- Separation of a strongly adsorbed component
Equipment - Part 2
- Chromatography column and associated equipment
- Product recovery equipment
- Purification equipment
Startup
- Major parts of a typical production-scale liquid chromatography system
- Taking cuts
- Maintaining column level
- Startup procedure
Operation - Part 1
- Overview
- Operating procedures
Operation - Part 2
- Charging feed to the column
- Washing out salt, solvent, and other impurities
- Eluting adsorbed components
- Taking cuts
- Taking product samples
Regeneration - Part 1
- The need for regeneration
- Major steps in regeneration
Regeneration - Part 2
- Adding regenerant and removing adsorbed eluant
- Washing regenerant out of the column
- Backwashing the column
- Returning the column to operation
- Replacing spent resin
|
|
|