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FERMENTATION
(COFER)
Format: Video
O V E R V I E W
Fermentation introduces trainees to the general operation of fermentation systems. After completing this unit, trainees should be able to identify the components in a typical fermentation system and explain how a production culture is prepared and how raw materials are used in fermenta¬tion processes. In addition, trainees should be able to describe operator responsibilities associated with inoculating, incubating, and harvesting fermenters. Trainees should also be able to describe the general steps involved in product recovery.
O J E C T I V E S
Introduction to Fermentation
- Define fermentation, microbe, binary fission, inoculation, inoculum, and incubation.
- Identify and describe different types of microbes.
- Explain how fermentation occurs in milk.
- Describe the stages of the microbe growth cycle.
- Describe the operation of a simple fermentation system.
Preparing a Production Culture
- Define pure culture and production culture.
- Describe how a pure culture and a production culture can be obtained.
- Describe how a production culture can be stored.
- Describe one method of preparing a production culture.
Raw Materials
- Identify raw materials commonly used in fermentation.
- Describe preparations associated with charging raw materials to seed tanks or fermen¬tors.
Fermentor Systems and Charging
- Identify the major components of a typical fermentor and describe their functions.
- Describe the following methods of fermentor operation: continuous, semi-continuous, and batch.
- Describe the charging and sterilization of a typical fermentor.
Fermentation Operations
- Describe operator responsibilities associated with inoculating a typical seed tank and a typical fermentor.
- Describe operator responsibilities during the incubation step and the harvesting step.
Product Recovery
- Describe the general steps involved in extracellular product recovery and in intracellular product recovery.
- Describe an example of a product recovery system.
S U B J E C T S
Introduction to Fermentation
- Fermentation and microbes
- How fermentation works: Milk example
- Microbe growth cycle
- Operation of a simple fermentation system
Preparing a Production Culture
- Obtaining a culture for production
- Storing a production culture
- Preparing a production culture for use
Raw Materials
- Nutrients supplied by raw materials
- Other additives
- Preparing raw materials for charging of seed tanks and fermentors
Fermentor Systems and Charging
- Fermentor components and operation
- Methods of fermentation
- Fermentor charging and sterilization
Fermentation Operations
- Inoculation
- Seed tank inoculation
- Fermentor inoculation
- Incubation
- Harvesting
Product Recovery
- Types of products
- Extracellular product recovery
- Intracellular product recovery
- Product recovery system example
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